Bacteriological Monitoring

Bacteriological monitoring is focused on detecting concentrations of E. coli and other bacteria in water, which serve as indicators of contamination. Monitors are trained in bacteriological testing, water quality standards, plate techniques, bacterial counts and site selection. Data from this type of monitoring is compiled in the statewide, citizens database. Training is conducted in two hour sessions where citizens are trained in bacteriological testing for E. coli and other coliform bacteria. Workshop objectives include:

  • An introduction to bacteriological testing and water quality standards
  • Demonstration of plate techniques and bacterial counts
  • Discussion of site selection.

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Monitoring Supplies

Bacteria Test Kit (per sample – 3 replicates)
Approximately $7.60 – $8.50 (depending on quantity discounts and shipping charges)

A test kit includes enough supplies to collect three replicate samples at each sampling site. The average of three replicates provides a much more reliable estimate of the actual number of bacterial colonies than a single sample.

Micrology Laboratories Inc
12441 Beckley St
Granger IN 46530

Contact: Doug Wengerd

Phone: 888-327-9435
NEW Phone: 574-970-3174
NEW Fax: 574-970-3170

ColiScan EasyGel Media & Pre-treated Petri Dishes (Catalog no. 25001)
$2.75 – $3.00 per replicate (depending on quantity discounts and shipping charges)

1-mL sterile-wrapped pipette (Catalog no. DRP01)
$0.23 each

The incubator for culturing bacterial samples can be made for less than $10. A small cooler or other type of insulated container with a lid may be used as an incubator. Uniform temperatures may be maintained using a small light bulb such as a night-light attached to an extension cord. Use a piece of cardboard or plastic to shield the sample plates from direct light. A thermostat is not needed. The number and/or wattage of bulbs may be adjusted to maintain the proper temperatures, depending on the ambient room temperature and size of incubator. For example, a 4-watt bulb in a 1 cubic foot container usually makes a good incubator. A thermometer is needed to determine ambient incubator temperature. This can be obtained from the AWW water chemistry test kit or a dial thermometer found in garden centers and hardware stores. The thermometer should be placed within the incubator away from the heat source or inserted through the lid so temperature may be read without opening the container.

Monitors can purchase high-quality, economic incubators from GQF Manufacturing Company the Hova-Bator Table Top Incubators.